Cryo-electron tomography EMD_5330

3D Model

Cryo-electron tomography reveals novel interactions and doublet-specific structures in the I1 dynein

Authors: Heuser T, Barber CF, Lin J, Krell J, Rebesco M, Porter ME, Nicastro D

Method: Subtomogram averaging (39 angstroms resolution) Red flagLatest update: 2014-04-02

Primary citation:

Cryoelectron tomography reveals doublet-specific structures and unique interactions in the I1 dynein.

Heuser T, Barber CF, Lin J, Krell J, Rebesco M, Porter ME, Nicastro D

PROC.NAT.ACAD.SCI.USA (2012) 109, pp. e2067-e2076 [PubMed 22733763] [DOI]

Sample: Cryo-electron tomography and subtomographic average (750 axonemal repeats) of isolated axonemes of wild type Chlamydomonas (CC 125, 137c), I1 dynein complex (dynein f) is bound to the doublet microtubule and is connected to neighboring structures.

Resolution: 39 Å (determined by FSC 0.5)

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