Microfluidic laser system

3D Model

A fluidic diffraction chip is developed to determine the amount of circulatingtumor cells (CTCs) or bacteria from human whole blood. We design the opticalstructure and transfer the pattern to transparent plastic substrate. The plasticdiffraction die is modified with antibodies of the target to combine a fluidicsystem as a fluidic diffraction chip. Human whole blood can be pumped into thefluidic diffraction chip directly without any pretreatment process to capture CTCsor bacteria. The captured CTCs or bacteria attach on the optical structureresulting in the linear change of diffraction intensity that can be read rapidly byusing the home-constructed laser beam system. Our platform has been appliedin the clinical diagnosis of oesophageal and endometrial cancer at Tri-ServiceGeneral Hospital and Taichung Veterans General Hospital, respectively, providinga novel method to count the CTCs/bacteria. Furthermore, the capturedCTCs/bacteria can be released from the chip after culture